Coding

Part:BBa_K259001:Design

Designed by: Petros Mina   Group: iGEM09_BCCS-Bristol   (2009-07-14)

fiu – Outer Membrane Ferric Iron Transporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 682
    Illegal AgeI site found at 820
    Illegal AgeI site found at 1225
    Illegal AgeI site found at 1999
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 724

Construction

This part was constructed by PCR amplification 

 Fwd Primer: 5'-GCTCTAGATGGAAAACAATCGCAATTTC-3'
 Rev Primer: 5'-GCACTAGTATTATTAGAAATGCATATTGGC-3'


Design Notes

In order to comply with assembly standard 10 (RFC10) the stop codon for fiu had to be changed to TAA.


Source

Fiu gene is part of the chromosomal E.coli genome. In particular it is found at 18.07min with orientation in the counter-clockwise direction.

[http://www.ecogene.org/geneInfo.php?eg_id=EG13317 Further details at EcoGene]

References